Sterilisation and keeping everything you use is important. Keep your work area clean and as sterile as possible. There is no reason to keep it as clean as an operating theatre just any thing that will come in to contact with semen and internally with the queen. keeping your tips and other glass wear clean you can use a standard pressure cooker see the download section for guidelines a lot of other site recommend using Autoclaves but this make things even more expensive and are not needed for the average Queen breeder.
A lot of the guidance given on other websites stems from notes given by university labs were they have to give scientific results at every stage of there research.
What we us here are a combination of the following.
- isopropyl alcohol
- Standard pressure cooker
- Ultrasonic Cleaner
You can get an Ultrasonic cleaner for around £20 it will not sterilize but it will dislodge hard particles of Semen and mucus i glass tips before sterilization.
An autoclave is a pressure chamber used to sterilize equipment and supplies by subjecting them to high pressure saturated steam at 121 °C (249°F) for around 15–20 minutes depending on the size of the load and the contents.